《植物生理学报》 2014, 50(12): 1857-1862

ABA喷施提高菊花抗蚜性及其生理机理

张瓒, 王萃铂, 房伟民, 陈发棣, 蒋甲福, 管志勇, 陈素梅*

南京农业大学园艺学院, 南京210095

通信作者：陈素梅；E-mail: chensm@njau.edu.cn；Tel: 025-84399670

摘　要：

为了检测ABA对菊花抗蚜性的影响, 以切花菊‘神马’为材料, 叶面喷施浓度为0 (对照)、0.06和0.1 mmol•L^-1脱落酸(abscisic acid, ABA), 24 h后接种蚜虫。接种7 d开始统计蚜虫数目, 每2 d统计一次, 连续统计2周, 发现0.06 mmol•L^-1 ABA处理下, 蚜虫数目明显少于对照和0.1 mmol•L^-1 ABA处理, 即0.06 mmol•L^-1 ABA可以提高菊花的抗蚜性。同时研究了外源ABA以及蚜虫接种后菊花叶片的抗氧化酶SOD、POD、CAT活性、H₂O₂含量和超氧阴离子产生速率。结果表明: 未接种蚜虫时, 0.06 mmol•L^-1 ABA处理下各生理指标均有升高, 而0.1 mmol•L^-1 ABA处理与对照无显著差异(P<0.05)。0.06 mmol•L^-1 ABA预处理后接种蚜虫植株中, SOD、POD和CAT活性表现为先上升后下降再上升的趋势, 峰值分别为对照的1.6、2.3和1.9倍, H₂O₂含量和超氧阴离子产生速率具有类似的变化趋势, 峰值时均为对照的2.3倍, 与对照相比均差异显著。表明ABA可以通过调节活性氧水平来提高菊花的抗蚜性。

关键词：脱落酸(ABA); 菊花; 蚜虫; 抗性

收稿：2014-10-22   修定：2014-11-26

资助：国家自然科学基金面上项目(31471900)、国家高技术研究发展计划(863计划) (2011AA100208)、江苏省农业科技自主创新资金项目[CX(12)2020]和江苏省科技支撑计划(BE2012350和BE2011325)。

ABA Spraying Enhanced Aphids Resistance in Chrysanthemum and Corresponding Physiological Mechanisms

ZHANG Zan, WANG Cui-Bo, FANG Wei-Min, CHEN Fa-Di, JIANG Jia-Fu, GUAN Zhi-Yong, CHEN Su-Mei*

College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: CHEN Su-Mei; E-mail: chensm@njau.edu.cn; Tel: 025-84399670

Abstract:

In order to testify the effects of ABA on the aphid resistance, ABA (0, 0.06 and 0.1 mmol•L^-1) was applied to the chrysanthemum ‘Jinba’ by foliar spray, followed by aphid inoculation. The number of aphids were counted 7 days after inoculation. The results showed that 0.06 mmol•L^-1 ABA could improve aphids resistance in chrysanthemum compared with 0.1 mmol•L^-1ABA and control (0 mmol•L^-1 ABA). We investigated the activities of the antioxidant enzyme (SOD, POD and CAT), content of the hydrogen peroxide (H₂O₂) and superoxide anion (O2.–) producing rate. Under 0.06 mmol•L^-1 ABA treatment the indicators rose compared with the control. In contrast, there were no differences in changes of the investigated indicator compaered with the control under 0.1 mmol•L^-1 ABA (P<0.05). The results demonstrated that the activities of SOD, POD and CAT rose in the early stage, then decreased and followed by an increase at the late phase of the treatment, and peaked to 1.6, 2.3 and 1.9 times, respectively, that of the control. Meanwhile, similar trends in changes were observed in the content of H₂O₂ and O2.– producing rate. It was illustrated that there were a concentration effect on improving aphids resistance in chrysanthemum with ABA.

Key words: abscisic acid (ABA); chrysanthemum; aphid; resistance